当归多糖的研究进展

张玉, 王凯平

中国药学杂志 ›› 2023, Vol. 58 ›› Issue (12) : 1065-1073.

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PDF(1021 KB)
中国药学杂志 ›› 2023, Vol. 58 ›› Issue (12) : 1065-1073. DOI: 10.11669/cpj.2023.12.002
综述

当归多糖的研究进展

  • 张玉1, 王凯平2*
作者信息 +

Research Progress of Angelica sinensis Polysaccharide

  • ZHANG Yu1, WANG Kai-ping2*
Author information +
文章历史 +

摘要

当归作为传统的药食同源的中药材,素有“十方九归”之美誉,具有补血活血、调经止痛、保肝护肝等功效。当归多糖是其最主要的活性成分之一,现代药理研究表明,当归多糖具有抗贫血、保肝护肝、调节糖脂代谢等多种药理活性。本文系统性总结了当归多糖的提取分离、结构解析、药动学和药理作用等方面的研究,旨在为当归多糖的后续深入研究提供科学有效的理论基础与依据。

Abstract

As a traditional Chinese medicinal material and food, Angelica sinensis is known because of its effects like nourishing blood and activating blood, regulating menstruation and relieving pain, and protecting the liver. Angelica sinensis polysaccharide is one of the most important active ingredients, modern pharmacological studies have shown that Angelica sinensis polysaccharide has the improvement of anemia, liver protection, regulation of glycolipid metabolism and other pharmacological activities. This review systematically summarizes the research on the extraction and isolation, structure elucidation, pharmacokinetics and pharmacological effects of Angelica sinensis polysaccharide, aiming to provide a scientific and effective theoretical basis for the subsequent study of Angelica sinensis polysaccharide.

关键词

当归多糖 / 分离 / 纯化 / 化学结构 / 药动学 / 药理活性 / 制剂

Key words

Angelica sinensis polysaccharide / isolation / purification / chemical structure / pharmacokinetics / pharmacological activity / preparation

引用本文

导出引用
张玉, 王凯平. 当归多糖的研究进展[J]. 中国药学杂志, 2023, 58(12): 1065-1073 https://doi.org/10.11669/cpj.2023.12.002
ZHANG Yu, WANG Kai-ping. Research Progress of Angelica sinensis Polysaccharide[J]. Chinese Pharmaceutical Journal, 2023, 58(12): 1065-1073 https://doi.org/10.11669/cpj.2023.12.002
中图分类号: R284   

参考文献

[1] LU C L, LI H H, SHI Y H, et al. Research progress of Angelicae Sinensis Radix and predictive analysis on its quality markers[J]. China J Chin Mater Med(中国中药杂志), 2022, 47(19):5140-5157.
[2] YU F, LI H, Meng Y, et al. Extraction optimization of Angelica sinensis polysaccharides and its antioxidant activity in vivo[J]. Carbohydr Polym, 2013, 94(1):114-119.
[3] ZHANG Y, ZHOU T, WANG H, et al. Structural characterization and in vitro antitumor activity of an acidic polysaccharide from Angelica sinensis (Oliv.) Diels[J]. Carbohydr Polym, 2016, 147:401-408.
[4] ZHAO Y, SHI Y, YANG H, et al. Extraction of Angelica sinensis polysaccharides using ultrasound-assisted way and its bioactivity[J]. Int J Biol Macromol, 2016, 88:44-50.
[5] ZHANG S B, LV J X. Comparison on four extraction methods of polysaccharide from Angelica sinensis [J]. Tianjin Agric Sci Res (天津农业科学), 2013, 19(10):33-36.
[6] JIN L C, LI G W, MA S L. Study on the Preparation technology of Angelica sinensis polysaccharides [J]. Chin Tradit Pat Med (中成药), 2007,29(8):1146-1150.
[7] WANG Y, LI X, ZHAO P, et al. Physicochemical characterizations of polysaccharides from Angelica Sinensis Radix under different drying methods for various applications[J]. Int J Biol Macromol, 2019, 121:381-389.
[8] CAO W, LI XQ, HOU Y, et al. Structural analysis and anti-tumor activity in vivo of polysaccharide APS-2a from Angelica sinensis [J]. J Chin Med Mater(中药材), 2008, 31(2):261-266.
[9] YANG X, ZHAO Y, LV Y. In vivo macrophage activation and physicochemical property of the different polysaccharide fractions purified from Angelica sinensis [J]. Carbohydr Polym, 2008, 71(3):372-379.
[10] SUN Y L, GU X H, TANG J, et al. The preparation, purification and structure analysis of water-soluble polysaccharides from Angelica sinensis[J]. J Food Biochem Technol(食品与生物技术学报), 2006(1):1-4.
[11] YANG X, ZHAO Y, LI G, et al. Chemical composition and immuno-stimulating properties of polysaccharide biological response modifier isolated from Radix Angelica sinensis[J]. Food Chem, 2008, 106(1):269-276.
[12] WANG S C, SHI S S, CUI J, et al. Isolation and structure analysis of a hetero-polysaccharide from Angelica sinensis (oliv.) diels[J]. Chin Pharm J(中国药学杂志), 2007,42(16):1255-1258.
[13] YANG X, YAN Z, YOU L. In vivo macrophage activation and physicochemical property of the different polysaccharide fractions purified from Angelica sinensis [J]. Carbohyd Polym, 2008, 71(3):372-379.
[14] CHEN X, CAO W, SUN Y, et al. Structural Characteristics and In Vitro Antitumor Effects of Angelica sinensis Polysaccharide APS-bⅡ[J]. Sci Technol Eng (科学技术与工程), 2010, 10(8):1839-1843.
[15] CAO W, LI X Q, WANG X, et al. Characterizations and anti-tumor activities of three acidic polysaccharides from Angelica sinensis (Oliv.) Diels[J]. Int J Biol Macromol, 2010, 46(1):115-122.
[16] SUN Y, CUI S W, JIAN T, et al. Structural features of pectic polysaccharide from Angelica sinensis (Oliv.) Diels[J]. Carbohyd Polym, 2010, 80(2):544-550.
[17] PAN S, JIANG L, WU S. Stimulating effects of polysaccharide from Angelica sinensis on the nonspecific immunity of white shrimps (Litopenaeus vannamei)[J]. Fish Shellfish Immunol, 2018, 74:170-174.
[18] SHAN J J, WANG Y, WANG S C, et al. Effect of Angelica sinensis polysaccharides on lymphocyte proliferation and induction of IFN-gamma [J]. Acta Pharm Sin(药学学报), 2002, 37(7):497-500.
[19] WANG J, GE B, LI Z, et al. Structural analysis and immunoregulation activity comparison of five polysaccharides from Angelica sinensis[J]. Carbohydr Polym, 2016, 140:6-12.
[20] CAO W, LI X Q, WANG X, et al. A novel polysaccharide, isolated from Angelica sinensis (Oliv.) Diels induces the apoptosis of cervical cancer HeLa cells through an intrinsic apoptotic pathway[J]. Phytomedicine, 2010, 17(8-9):598-605.
[21] CAO W, LI X Q, LIU L, et al. Structural analysis of water-soluble glucans from the root of Angelica sinensis (Oliv.) Diels[J]. Carbohydr Res, 2006, 341(11):1870-1877.
[22] ZHAO L, WANG Y, SHEN H L, et al. Structural characterization and radioprotection of bone marrow hematopoiesis of two novel polysaccharides from the root of Angelica sinensis (Oliv.) Diels[J]. Fitoterapia, 2012, 83(8):1712-1720.
[23] LIU W, LI W, SUI Y, et al. Structure characterization and anti-leukemia activity of a novel polysaccharide from Angelica sinensis (Oliv.) Diels[J]. Int J Biol Macromol, 2019, 121:161-172.
[24] SUN Y, TANG J, GU X, et al. Water-soluble polysaccharides from Angelica sinensis (Oliv.) Diels:Preparation, characterization and bioactivity[J]. Int J Biol Macromol, 2005, 36(5):283-289.
[25] MA P, SUN C, LI W, et al. Extraction and structural analysis of Angelica sinensis polysaccharide with low molecular weight and its lipid-lowering effect on nonalcoholic fatty liver disease[J]. Food Sci Nutr, 2020, 8(7):3212-3224.
[26] ZHOU T. Isolation, structural identification and biological activity of polysacharides from Angelica sinensis[D]. Wuhan: Huazhong University of Science and Technology, 2018.
[27] LIU W, XIAO K, REN L, et al. Leukemia cells apoptosis by a newly discovered heterogeneous polysaccharide from Angelica sinensis (Oliv.) Diels[J]. Carbohydr Polym, 2020, 241:116279.
[28] ZENG Q H, ZHANG X W, XU K P, et al. Application of fluorescently labeled tracer technique for detection of natural active macromolecules in Chinese medicine[J]. Drug Metab Rev, 2014, 46(1):57-71.
[29] MECHREF Y, HU Y, DESANTOS-GARCIA J L, et al. Quantitative glycomics strategies[J]. Mol Cell Proteom, 2013, 12(4):874-884.
[30] DE GRAAF R A, THOMAS M A, BEHAR K L, et al. Characterization of kinetic isotope effects and label loss in deuterium-based isotopic labeling studies[J]. ACS Chem Neurosci, 2021, 12(1):234-243.
[31] ZHANG Y, ZHOU T, LUO L, et al. Pharmacokinetics, biodistribution and receptor mediated endocytosis of a natural Angelica sinensis polysaccharide[J]. Artif Cells Nanomed Biotechnol, 2018, 46(sup1):254-263.
[32] ZOCOLA E, MWYER J, CHRISTOU N, et al. Role of near-infrared fluorescence in colorectal surgery[J]. World J Gastroenterol, 2021, 27(31):5189-5200.
[33] WANG K P, CHENG F, PAN X L, et al. Investigation of the transport and absorption of Angelica sinensis polysaccharide through gastrointestinal tract both in vitro and in vivo[J]. Drug Deliv, 2017, 24(1):1360-1371.
[34] WANG K P, ZENG F, LIU J Y, et al. Inhibitory effect of polysaccharides isolated from Angelica sinensis on hepcidin expression[J]. J Ethnopharmacol, 2011, 134(3):944-948.
[35] ZHANG Y, CHENG Y, WANG N, et al. The action of JAK, SMAD and ERK signal pathways on hepcidin suppression by polysaccharides from Angelica sinensis in rats with iron deficiency anemia[J]. Food Funct, 2014, 5(7):1381-1388.
[36] LIU J Y, ZHANG Y, YOU R X, et al. Polysaccharide isolated from Angelica sinensis inhibits hepcidin expression in rats with iron deficiency anemia[J]. J Med Food, 2012, 15(10):923-929.
[37] WANG K, WU J, CHENG F, et al. Acidic polysaccharide from Angelica sinensis reverses anemia of chronic disease involving the suppression of inflammatory hepcidin and NF-kappa B activation[J]. Oxid Med Cell Longev, 2017, 2017:7601592.
[38] LI M M, ZHANG Y, WU J, et al. Polysaccharide from Angelica Sinensis suppresses inflammation and reverses anemia in complete freund's adjuvant-induced rats[J]. Curr Med Sci, 2020, 40(2):265-274.
[39] WANG K, WU J, XU J, et al. Correction of anemia in chronic kidney disease with Angelica sinensis polysaccharide via restoring EPO production and improving iron availability[J]. Front Pharmacol, 2018, 9:803.
[40] ZHANG X P, LIU J, XU C Y, et al. Effect of Angelica sinensis polysaccharide on expression of telomere,telomerase and P53 in mice aging hematopoietic stem cells [J]. China J Chin Mater Med(中国中药杂志),2013,38(14):2354.
[41] LI H H, TANG S H, ZHANG X P, et al. Effect of Angelica sinensis polysaccharide on expression of SIRT1, p53 and p21 in hematopoietic stem cells [J]. Guangdong Med J(广东医学),2021, 42(12):1437.
[42] CHEN Z, CHENG L, ZHANG J, et al. Angelica sinensis polysaccharide prevents mitochondrial apoptosis by regulating the Treg/Th17 ratio in aplastic anemia[J]. BMC Complement Med Ther, 2020, 20(1):192.
[43] COLLABORATORS G R F.Global, regional, and national comparative risk assessment of 84 behavioural, environmental and occupational, and metabolic risks or clusters of risks, 1990-2016:a systematic analysis for the Global Burden of Disease Study 2016[J]. Lancet, 2017, 390(10100):1345-1422.
[44] ZHANG Y, WAN X, YANG F, et al. Protective Effect of Angelica Polysaccharide on Alcoholic Liver Injury in Mice[J]. J Changzhi Med Coll(长治医学院学报), 2019, 33(3):165-168.
[45] CAO P, ZHANG Y, WANG H X, et al. Preventive effect of Angelica sinensis polysaccharide on acute alcoholic liver injury in mice and its influence on HIF-1α protein [J]. China J Tradit Chin Med Pharm(中华中医药杂志), 2021, 36(6):3628-3631.
[46] CAO P, SUN J, Sullivan M A, et al. Angelica sinensis polysaccharide protects against acetaminophen-induced acute liver injury and cell death by suppressing oxidative stress and hepatic apoptosis in vivo and in vitro[J]. Int J Biol Macromol, 2018, 111:1133-1139.
[47] FU Z Y, MA C X. Effect of Angelica Polysaccharide on liver injury induced by cisplatin chemotherapy in H22 ascites tumor mice[J]. Pharmacol Clin Chin Mater Med(中药药理与临床), 2018, 34(3):68-72.
[48] WANG K P, SONG Z Z, WANG H X, et al. Angelica sinensis polysaccharide attenuates concanavalin A-induced liver injury in mice[J]. Int Immunopharmacol, 2016, 31:140-148.
[49] YU F. Protective Effects of Angelica sinensis polysaccharide on Immunological Liver Injury Induced by Concanavalin A in Mice[J]. J Chin Med Mater(中药材), 2009, 32(10):1580-1583.
[50] WANG K P, WANG J F, SONG M Z, et al. Angelica sinensis polysaccharide attenuates CCl4-induced liver fibrosis via the IL-22/STAT3 pathway[J]. Int J Biol Macromol, 2020, 162:273-283.
[51] HE Z H, GUO T T, CUI Z, et al. New understanding of Angelica sinensis polysaccharide improving fatty liver:The dual inhibition of lipid synthesis and CD36-mediated lipid uptake and the regulation of alcohol metabolism[J]. Int J Biol Macromol, 2022, 207:813-825.
[52] MA P, SUN C Y, LI W J, et al. Extraction and structural analysis of Angelica sinensis polysaccharide with low molecular weight and its lipid-lowering effect on nonalcoholic fatty liver disease[J]. Food Sci Nutr, 2020, 8(7):3212-3224.
[53] KIM H M, KANG J S, PARK S, et al. Antidiabetic activity of angelan isolated from Angelica gigas Nakai[J]. Arch Pharm Res, 2008, 31(11):1489-1496.
[54] WANG K P, CAO P, SHUI W Z, et al. Angelica sinensis polysaccharide regulates glucose and lipid metabolism disorder in prediabetic and streptozotocin-induced diabetic mice through the elevation of glycogen levels and reduction of inflammatory factors[J]. Food Funct, 2015, 6(3):902-909.
[55] ZHANG Y, HE Z H, LIU X C, et al. Oral administration of Angelica sinensis polysaccharide protects against pancreatic islets failure in type 2 diabetic mice:Pancreatic β-cell apoptosis inhibition[J]. J Funct Foods, 2019, 54:361-370.
[56] LI C J, CHEN P. Lowering Sugar Effect and Mechanism of Angelica Polysaccharide to Wister Diabetic Rats Induced by STZ[J]. J Qiqihar Med Univ(齐齐哈尔医学院学报), 2007(10):1158-1161.
[57] WANG K P, TANG Z H, ZHENG Z M, et al. Protective effects of Angelica sinensis polysaccharide against hyperglycemia and liver injury in multiple low-dose streptozotocin-induced type 2 diabetic BALB/c mice[J]. Food Funct, 2016, 7(12):4889-4897.
[58] MENG X Y, ZHANG Z H, WANG Y F. Influence of Angelica Polysaccharide on Lipid Metabolism and Adipokine in Diabetic Rats[J]. West J Tradit Chin Med(西部中医药), 2021, 34(11):33-36.
[59] SUI Y, LIU W J, TIAN W, et al. A branched arabinoglucan from Angelica sinensis ameliorates diabetic renal damage in rats[J]. Phytother Res, 2019, 33(3):818-831
[60] ZHOU X H, WANG Q L, ZHU X M, et al. Effects of Angelica sinensis polysaccharide on TLR4/MyD88/NF-κB pathway in rats with diabetic neuropathy[J]. Chin J Clin Pharmacol(中国临床药理学与治疗学), 2018, 23(12):1340-1347.
[61] LIAO F, LIU Y, LIU H H, et al. Effect of Angelica sinensis polysaccharide on the osteogenic differentiation of bone marrow mesenchymal stem cells of rats with high glucose levels[J]. West China J Stomatol(华西口腔医学杂志), 2019, 37(2):193-199.
[62] SHI Y Q. Pharmacological Mechanism and Effects of Angelica Polysaccharide on Diabetic Nephropathy Based on TGF-β1/Smads Signaling Pathway[D]. Lanzhou:Lanzhou University, 2018.
[63] LI X Y, LIU Z Z. Effects of Angelica sinensis polysaccharides on the immunological funtion of the rats with colitis [J]. Pract Clin Med(实用临床医学), 2005, 6(7):11-16.
[64] PAN Q H, LI Y Z, LIU D Y, et al. Effects of Angelica sinensis polysaccharide on regulation of T lymphocyte subsets in Peyer's patches in mice with colitis [J]. Jiangxi J Tradit Chin Med(江西中医药), 2015, 46(10):37-46.
[65] CHENG F, ZHANG Y, LI Q, et al. Inhibition of Dextran Sodium Sulfate-Induced Experimental Colitis in Mice by Angelica Sinensis Polysaccharide[J]. J Med Food, 2020, 23(6):584-592.
[66] CHENG Y, WANG K P, LI Q, et al. Effects of polysaccharide from Angelica sinensis on tumor growth and iron metabolism in vivo in H22-bearing mice [J]. Chin J Hosp Pharm(中国医院药学杂志), 2015, 35(15):1359.
[67] CAO W, LI X Q, HOU Y, et al. Physicochemical Properties and Anti-tumor Activity in vitro of Polysaccharide APS-3c from Angelica sinensis (Oliv.) Diels [J]. Nat Prod Res Dev(天然产物研究与开发), 2008(2):217.
[68] ZHOU W J, WANG S, HU Z, et al. Angelica sinensis polysaccharides promotes apoptosis in human breast cancer cells via CREB-regulated caspase-3 activation[J]. Biochem Biophys Res Commun, 2015, 467(3):562-569.
[69] QIN T, CHEN J, WANG D Y, et al. Selenylation modification can enhance immune-enhancing activity of Chinese Angelica polysaccharide[J]. Carbohyd Polym, 2013, 95(1):183-187
[70] WANG S F, WANG X, GUI X E, et al. Effects of Angelica polysaccharide on the functional status of dendritic cells in HBC trans-genic mice[J]. J Chin Pract Diagn Ther(中华实用诊断与治疗杂志), 2005(5):313-317.
[71] ZHANG P P, WANG Q W, CHEN H Q, et al. In Vitro Suppressive Effect of Angelica Polysaccharide on Human Cytomegalovirus-induced Apoptosis via Direct Infection in CHRF-288-11 Cells[J]. J Exp Hematol(中国实验血液学杂志), 2009, 17(1):193-197
[72] WANF K P, ZHANG Y, DAI L Q, et al. Preliminary studies on physicochemical properties of Angelica sinensis polysaccharide-iron complex [J]. China J Chin Mater Med(中国中药杂志), 2006(9):747-750.
[73] WAGN K P, CHEN Z X, ZHANG Y, et al. Experimental study of Angelica sinensis polysaccharide-iron complex on rats with iron deficiency anemia [J]. Chin J New Drugs Clin Rem(中国新药与临床杂志), 2007(6):413-416.
[74] SHI C, ZHANG Y, WANG K P. Preparation of the Angelica sinensis polysaccharide-iron complex floating sustained-release tablets and evaluation of its pharmacokinetics in dogs [J]. Chin J Hosp Pharm (中国医院药学杂志), 2011, 31(5):365-369.
[75] QIN L, LI P, YANG Q M, et al. Study on the preparation process of Danggui Baogan Capsules [J]. China Pharm(中国药师), 2016, 19(3):480-483.
[76] DENG W, FU M, CAO Y, et al. Angelica sinensis polysaccharide nanoparticles as novel non-viral carriers for gene delivery to mesenchymal stem cells[J]. Nanomed Nanotechnol Biol Med, 2013, 9(8):1181-1191.
[77] WANG K, XU J, LIU Y, et al. Self-assembled Angelica sinensis polysaccharide nanoparticles with an instinctive liver-targeting ability as a drug carrier for acute alcoholic liver damage protection[J]. Int J Pharm, 2020, 577:118996.
[78] ZHANG Y, CUI Z, MEI H, et al. Angelica sinensis polysaccharide nanoparticles as a targeted drug delivery system for enhanced therapy of liver cancer[J]. Carbohyd Polym, 2019, 219:143.
[79] FANG L, LIN H, WU Z, et al. In vitro/vivo evaluation of novel mitochondrial targeting charge-reversal polysaccharide-based antitumor nanoparticle[J]. Carbohyd Polym, 2020, 234:115930.
[80] GUO C, HOU X, LIU Y, et al. Novel Chinese Angelica Polysaccharide Biomimetic Nanomedicine to Curcumin Delivery for Hepatocellular Carcinoma Treatment and Immunomodulatory Effect[J]. Phytomedicine, 2021, 80:153356.
[81] GU P, LIU Z, SUN Y, et al. Angelica sinensis polysaccharide encapsulated into PLGA nanoparticles as a vaccine delivery and adjuvant system for ovalbumin to promote immune responses[J]. Int J Pharm, 2019, 554:72-80.
[82] GU P, WUSIMAN A, WANG S, et al. Polyethylenimine-coated PLGA nanoparticles-encapsulated Angelica sinensis polysaccharide as an adjuvant to enhance immune responses[J]. Carbohyd Polym, 2019, 223:115128.

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国家自然科学基金项目资助(81974509,82074111)
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